The first half was goalless, but straight after half-time, Ivan Perišić made his mark as he netted, with an assist by Noa Lang, to give PSV the lead.
In the 63rd minute, Timothy Weah equalised for Juventus.
The 3-1 goal came in the final minutes, when Ryan Flamingo found the net for PSV.
The previous match between the teams ended in a 2-1 win for Juventus on home soil.
Juventus almost lasted the distance away to PSV in the Champions League, the home side needed extra time to take a 3-1 win.
Wrapping up the regular season, PSV finished in 14th place and Juventus in 20th.
Ismael Saibari gave PSV the lead in the 74th minute with an assist by Luuk de Jong.
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